Optimal culture incubation time in orthopedic device-associated infections: a retrospective analysis of prolonged 14-day incubation.

Optimal culture incubation time in orthopedic 1 device - associated infections – a retrospective 2 analysis of prolonged 14 - day incubation

Propionibacterium acnes: Time-to-Positivity in Standard Bacterial Culture From Different Anatomical Sites

BACKGROUND Propionibacterium acnes infections are likely under-recognized and underreported. This is partly because of low clinical suspicion, perceived non-pathogenicity, or lack of adequate culture incubation time. We conducted a study to assess the optimal incubation period to recover P. acnes from specimens acquired during the workup of suspected clinical infections. METHODS A 5-year retr...

Use of an automated blood culture system (BD BACTEC™) for diagnosis of prosthetic joint infections: easy and fast

BACKGROUND For the diagnosis of prosthetic joint infection (PJI) automated BACTEC™ blood culture bottle methods have comparable sensitivity, specificity and a shorter time to positivity than traditional cooked meat enrichment broth methods. We evaluate the culture incubation period required to maximise sensitivity and specificity of microbiological diagnosis, and the ability of BACTEC™ to detec...

One-Factor-at-a-Time Optimization of Polyhydroxybutyrate Production and Growth of Alcaligenes eutrophus by Altering Culture Parameters and Incubation Time

Polyhydroxyalkanoates (PHAs) are bioplastics derived from renewable resources such as vegetable oils, corn starch, or microbes. The polyhydroxybutyrate (PHB) is a short-chain-length PHA, and the most important bioplastic produced by certain microorganisms in the presence of excess carbon sources. In this study batch cultivation of Alcaligenes eutrophus with the aim of increasing PHB production ...

Improved diagnosis of orthopedic implant-associated infection by inoculation of sonication fluid into blood culture bottles.

Sonication improved the diagnosis of orthopedic implant-associated infections (OIAI). We investigated the diagnostic performance of sonication fluid inoculated into blood culture bottles in comparison with that of intraoperative tissue and sonication fluid cultures. Consecutive patients with removed orthopedic hardware were prospectively included and classified as having OIAI or aseptic failure...